5x HOT FIREPol® Evagreen® qPCR Supermix..1ml; 250 rxns



5x HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.

The Mix contains HOT FIREPol® DNA Polymerase and an passive internal reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiering platforms.

The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.


  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination


Concentration: 5x

Hot-start: yes, initial activation in 12 min

Detection type: dye-based, includes EvaGreen® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: most qPCR cyclers. Check Your cycler!

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start

5x EvaGreen® qPCR buffer with 12. 5 mM MgCl21x PCR solution – 2.5 mM MgCl2

dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix

EvaGreen® dye

Internal reference based on ROX dye


Blue visualisation dye

EvaGreen Dye

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

15 in stock (can be backordered)

Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting

Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results, and demonstrates excellent performance with GC-rich regions.

  • high sensitivity with low DNA concentrations
  • blue dye to ease pipetting
  • reduced primer dimer formation
  • one qPCR mix for all cyclers (except capillary)
  • contains dUTP to prevent cross-contamination with UNG treatment


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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.


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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)