Hot-start: yes, initial activation in 12 min
Detection type: dye-based, includes EvaGreen® intercalating dye
Reference dye: based on ROX
Compatible real-time instruments: most qPCR cyclers. Check Your cycler!
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix
Internal reference based on ROX dye
Blue visualisation dye
EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!