5x HOT FIREPol® MultiPlex Mix with 10 mM MgCl2 RTL 1ml


Hot-start master mix for high throughput applications with ready-to-load feature

  • analyze multiple targets per reaction
  • increased sensitivity and yield
  • prevents primer dimer formation
  • reaction set-up and shipment without dry ice

4 in stock (can be backordered)


5x HOT FIREPol® MultiPlex Mix Ready to Load  is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water) and an additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

Generated PCR products are compatible with T/A cloning procedures.


We recommend using HOT FIREPol® MultiPlex Mix Ready To Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

HOT FIREPol® MultiPlex Mix Ready To Load is not recommended for use in applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.


Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Ready to load: yes

Fidelity: 1 x Taq

Cloning Type: T/A cloning

Multiplexing: tested with up to 18 targets (depending on amplicon length)

Amplicon Size: up to 5 kb

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start

5x Multiplex Buffer with 10 mM MgCl21x PCR solution – 2 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP

Bovine serum albumine (BSA) to enhance PCR reaction

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading 


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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.


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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)