5x HOT FIREPol® Probe qPCR Mix Plus (no ROX) Cat 08-15-00001

30,000.00 39,000.00

Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry

Cost-effective real time qPCR master mix for probe-based qPCR assays.
This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well.

  • suitable for singleplex and duplex assays
  • high specificity and sensitivity
  • reaction set-up and shipment without dry ice

4 in stock


HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.

The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.


  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination


Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Detection type: Probe-based

Reference dye: none

Compatible real-time instruments: cyclers that do not need ROX reference dye. Check Your cycler!

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start

5x Probe qPCR buffer with 15 mM MgCl21x PCR solution – 3 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP


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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.


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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)