Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Ethidium Bromide solution is a fluorescent stain for visualizing nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0.625mg/mL. A final working concentration of 0.5g/mL is easily obtained by adding 1 drop of solution to 50 mL of molten agarose, running buffer or staining buffer. Ethidium Bromide solution in dropper bottles may be stored at room temperature for up to 1 year.
Dropper bottles are shatter-resistant and light-proof. No additional hazardous shipping costs are applied to Ethidium Bromide Dropper Bottles. One drop per 50 mL of water equals 0.5ug/mL Ethidium Bromide
Frameshift mutagen which intercalates double-stranded DNA and RNA.