Ethidium Bromide

39,200.00 42,500.00

Ethidium Bromide 0.625 mg/dl

E406-5ml

1 in stock

SKU: SKUEtBr Categories: ,

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

 

Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

Ethidium Bromide solution is a fluorescent stain for visualizing nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0.625mg/mL. A final working concentration of 0.5g/mL is easily obtained by adding 1 drop of solution to 50 mL of molten agarose, running buffer or staining buffer. Ethidium Bromide solution in dropper bottles may be stored at room temperature for up to 1 year.

Dropper bottles are shatter-resistant and light-proof. No additional hazardous shipping costs are applied to Ethidium Bromide Dropper Bottles. One drop per 50 mL of water equals 0.5ug/mL Ethidium Bromide

 

Frameshift mutagen which intercalates double-stranded DNA and RNA.

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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)