FIREPol 5x Master Mix 12.5 mM ready to load, 250 reactions; 1ml

19,800.00 32,000.00

Description

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.

Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

 

Properties

Concentration: 5x

Hot-start: no

Ready to load: yes

Fidelity: 1x Taq

Cloning Type: T/A Cloning

Amplicon Sixe: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading

26 in stock

Ready to load PCR Master Mix for routine applications

  • load directly to gel after PCR
  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time

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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.

 

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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)