Ready to load PCR Master Mix for routine applications
- load directly to gel after PCR
- suitable for templates up to 5 kb
- reaction set-up and shipment without dry ice
- all-in-one master mix format reduces pipetting errors and saves time
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.
Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.
Ready to load: yes
Fidelity: 1x Taq
Cloning Type: T/A Cloning
Amplicon Sixe: up to 5 kb
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
Yellow dye: migration equivalent to 35-45 bp DNA fragment
Compound that increases sample density for direct loading
10 in stock
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