Description

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol^®, MgCl₂, dNTPs and buffer with detergent. You just need to add template, primers and water.

Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

We recommend using 5x FIREPol^® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol^® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: no

Ready to load: yes

Fidelity: 1x Taq

Cloning Type: T/A Cloning

Amplicon Sixe: up to 5 kb

Mix Components

FIREPol^® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH₄)₂SO₄, 0.1% w/v Tween-20

7.5 mM MgCl_2: 1x PCR solution – 1.5 mM MgCl₂

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading