FIREPol 5x Master Mix 7.5 mM ready to load, 250 reactions; 1ml



Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.

Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.



Concentration: 5x

Hot-start: no

Ready to load: yes

Fidelity: 1x Taq

Cloning Type: T/A Cloning

Amplicon Sixe: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading

10 in stock (can be backordered)

Ready to load PCR Master Mix for routine applications

  • load directly to gel after PCR
  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time


There are no reviews yet.

Be the first to review “FIREPol 5x Master Mix 7.5 mM ready to load, 250 reactions; 1ml”
Review now to get coupon!

Your email address will not be published. Required fields are marked *

Shipping Details

  • Please pay more attention to your order address which MUST MATCH your shipping address. (If you’re from Russia, Please leave your full name. It is very important)
  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.


Shipping By Shipping Cost Estimated Delivery Time Tracking Information
Thembay Express Free Shipping 12-20 days Not available
LEX $20.00 - $50.00 04-12 days Available
Lorem Ex $26.00 - $70.00 03-17 days Available

Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)