QIAamp DNA Blood Mini Kit (50 Reactions) cat 51104

80,000.00 95,000.00

Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure “Apoptotic banding in stored blood“). The purified DNA is suitable for long-range PCR amplification (see figure “Long-range PCR“) and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure “Paternity testing by RFLP analysis“).

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

Procedure

The QIAamp DNA Blood Mini Kit simplifies isolation of DNA from blood and related body fluids with fast spin-column or vacuum procedures (see flowchart “procedure“). Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl, with a preparation time of 20–40 minutes. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. The QIAamp DNA Blood Mini Kit procedure is automatable (see figure “QIAcube“).

QIAamp sample preparation technology is fully licensed.

Vacuum processing

With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Applications

The QIAamp DNA Blood Mini Kit provides proven QIAamp technology for purification of DNA from a variety of materials using Mini spin columns with 2.0 ml collection tubes. Sample sources include:

Fresh and frozen whole blood or buffy coatPlasma or serumBone marrowLymphocytesPlateletsBody fluids

8 in stock (can be backordered)

SKU: RSV51104 Categories: ,
For purification of up to 12 µg genomic, mitochondrial, or viral DNA from blood and related body fluids
  • Rapid purification of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors for reliable results

The QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification. The QIAamp DNA Blood Mini Kit is designed for processing up to 200 μl fresh or frozen human whole blood. QIAamp Mini spin columns can be easily processed in a centrifuge or on vacuum manifolds

Reviews

There are no reviews yet.

Be the first to review “QIAamp DNA Blood Mini Kit (50 Reactions) cat 51104”
Review now to get coupon!

Your email address will not be published. Required fields are marked *

Shipping Details

  • Please pay more attention to your order address which MUST MATCH your shipping address. (If you’re from Russia, Please leave your full name. It is very important)
  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.

 

Shipping By Shipping Cost Estimated Delivery Time Tracking Information
Thembay Express Free Shipping 12-20 days Not available
LEX $20.00 - $50.00 04-12 days Available
Lorem Ex $26.00 - $70.00 03-17 days Available

Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)