RNA/DNA/Protein Purification Plus Kit 50 Preps

333,900.00

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RNA/DNA/Protein Purification Plus Kit

This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.

The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing silicon carbide columns (superior for the binding of all RNA sizes including miRNA).  The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application  without any further clean up required. The proteins can be quantified directly,  used in western blots, ELISA or mass spectrometry.

Background

This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.
The purified RNA, DNA and proteins are of the highest integrity and can be used in a number of downstream applications.

Protocol

Workflow

1 in stock (can be backordered)

SKU: 47700 Category:
  • Sequentially purify total RNA (and miRNA),  DNA and  proteins from a single sample
  • No sample splitting or need to use phenol or precipitation methods
  • Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
  • Rapid and efficient spin column procedure – all done in 30 minutes
  • Proteins are purified on column and are soluble in the elution buffer.  No further cleaning is required
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.

 

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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)