Taq FIREPol® DNA Polymerase 5U/ul (500U)

13,900.00 14,640.00

Highly processive, thermostable Taq DNA polymerase

Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results

  • robust amplification in routine applications
  • suitable for templates up to 5 kb
  • reaction set-up and shipment without ice
  • TA cloning

3 in stock

Description

FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month.

Recommended for routine applications (fragment up to 3 kb from genomic DNA).

Possesses 5′→3′ polymerase and 5′→3′ endonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.

The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5).

 

Properties

Concentration: 5 U/µl

Error rate per nucleotide per cycle is app. 2.5 x 10-5

Accuracy is app. 4 x 104

Estimated half life at 95ºC is 1.5 hours.

Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Kit Components

FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.

FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20.

FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.

FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4.

25 mM MgCl2

10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).

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Shipping Details

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  • Items will be shipped within 3 business days after payment.
  • Please check items when delivered, if damaged, please kindly accept it and contact us immediately.  We will make a confirmation and resend you a new one.

 

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Packaging Details

  • Unit Type: piece
  • Package Size: 25cm x 32cm x 5cm (9.84in x 12.60in x 1.97in)
  • Package Weight: 0.56kg (1.23lb.)